Taxonomic identification report

Sample ID: VE25-1406_COI

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[analysis description + link to github repo]

Facility Hogwarts
Analyst John Doe
Analysis started 2025-05-03 01:28:03
Analysis completed 2025-05-03 01:28:03
Wall time 0:0:0 hours

Result overview

Pteromalidae sp.

Outcome: Positive species identification.

Reasoning: [Flag 1A] 1 candidate species matched with high stringency (identity ≥ 98.5%).

Preliminary morphology ID confirmed? False

Flag 7B: Identified species is not consistent with preliminary morphology ID Muscidae.

Taxa of interest detected? False

Flag 2B: Taxon of interest NOT detected in candidate species

Flag 5.1A: The given locus for this taxon is well represented in reference database (>5 entries)

Flag 5.2C: ≤10% of taxon have reference sequence(s) at the given locus

Sample details

Locus COI
Preliminary ID Muscidae
Taxa of interest Atherigona orientalis
Country Vietnam
Host Dragon Fruit
Sample ID VE25-1406_COI
Query DNA sequence

Analyst evaluation

Identification of candidate species

Flag 1A: Positive species identification - Pteromalidae sp.
1 candidate species matched with high stringency (identity ≥ 98.5%)

Candidate hits must meet ONE of these criteria:

Minimum alignment length 400bp
Minimum query coverage 85.0%

Candidate hits have then been classified as follows:

Classification Alignment identity Number of hits Number of species
STRONG MATCH ≥ 98.5% 1 1
MODERATE MATCH ≥ 93.5% NA NA
NO MATCH < 93.5%

Candidate species

Species Hits Identity E-value Database coverage
Pteromalidae sp. 1 98.6% 0.0

Analyst evaluation

Taxa of interest (TOI)

This sections shows the taxa of interest (TOI) specified by the sample submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.

Taxon of interest Match rank Match taxon Match species Match accession Match identity Database coverage
Atherigona orientalis Did not match any candidate

Reference sequence source diversity

This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.

Analyst evaluation

Independent sources

Flag 4B: Reference sequence sources lack diversity and may therefore be unreliable
Reasoning: Matching sequence records for this species have only 1-5 independent sources (found 1 sources)

1 Independent Source

The matching reference sequences for this species have been annotated by 1 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession Automated Authors Title Journal
OQ134363 False Lee,S.
Park,D.-Y. 		Direct Submission Submitted (27-DEC-2022) Insect Biosystematics Laboratory, Seoul National University, Gwanak-ro 1, Seoul 08826, South Korea

Intraspecies diversity

This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.

Analyst evaluation

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The phylogetic tree was constructed with FastME using the Neighbor-Joining method. Multiple-sequence alignment of the candidate reference sequences was performed using MAFFT. The visualization is rendered with TidyTree.

Taxonomy Check

The following resources can be used to ensure that the given taxonomy is legitimate and current.

Taxa Database
General GBIF
General ITIS
Mealybugs & scale ScaleNet database
Thrips Thripswiki
Spider Mites Spider Mites Database
Psocodea (Barklice, Booklice, and Parasitic Lice) Psocodea Species File Online
Orthoptera Orthoptera Species File Online
Drosophilidae TaxoDros
Diptera Catalog of the Diptera of the Australasian and Oceanian Regions
Systema Dipterorum
Aphids Aphid Species File
Ants AntWeb
AntCat
Lepidoptera (butterflies and moths) The Global Lepidoptera Names Index
Gracillariidae (primitive moths) Global Taxonomic Database of Gracillariidae
Pyralidae (pyralid moths) Global Information System on Pyraloidea
Tortricidae (tortrix moths) Tortricidae Resources on the Net